اثر هشت هفته تمرین هوازی همراه با مکمل ویتامین E بر نشانگر های آپوپتوزی در بافت مغز موش های صحرایی مبتلا به پارکینسون

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران

2 گروه علوم ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران (نویسنده مسئول)

3 گروه علوم ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران

10.22038/mjms.2025.85277.4893

چکیده

هدف مطالعه حاضر بررسی اثر هشت هفته تمرین هوازی (AT) و مکمل ویتامین E (VE) بر Bax و Bcl-2 در بافت مغز موش‌های صحرایی مبتلا به پارکینسون بود.
روش کار: در این مطالعه تجربی 40 سر موش صحرایی نر نژاد اسپراگو-داولی (سن 14-16 ماه و وزن 250 تا 270 گرم) پارکینسونی شده mg/kg 2 رزرپین (Res) به گروه‌های (1) Res، (2) شم (Sh/حلال ویتامین E)، (3) AT، (4) VE و (5) AT+VE تقسیم شدند. جهت بررسی اثرات Res بر متغیر‌ها تعداد 8 سر موش صحرایی در گروه کنترل سالم (HC) قرار گرفتند. تمرین هوازی به مدت هشت هفته، پنج جلسه در هفته و هر جلسه مدت 15-48 دقیقه با سرعت 10-24 متر بر دقیقه انجام شد. VE نیز روزانه mg/kg 30 به صورت خوراکی مصرف شد. برای تجزیه و تحلیل داده‌ها از آزمون آنالیز واریانس یک‌راهه و آزمون تعقیبی توکی استفاده شد (05/0≥P).
نتایج: در گروه AT، VE و AT+VE مقادیر Bax و Bax/Bcl-2 پایین‌تر و مقادیر Bcl-2 بالاتر از گروه Res بود (001/0=P). همچنین مقادیر Bax در گروه VE (02/0=P) و AT+VE (001/0=P) به طور معنی‌داری کمتر از گروه AT بود؛ همچنین مقادیر Bcl-2 در گروه AT+VE به طور معنی‌داری بالاتر از گروه AT و VE بود (001/0=P).
نتیجه گیری به نظر می‌رسد تمرین هوازی و ویتامین E هم به تنهایی و هم به طور همزمان نقش مطلوبی در کاهش نشانگر‌های آپوپتوز بافت مغز در شرایط ابتلا به بیماری پارکینسون دارند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of eight weeks of aerobic training with vitamin E supplementation on apoptotic markers in the brain tissue of rates with Parkinson's disease

نویسندگان [English]

  • Fatemeh Sadeghi 1
  • Jamshid Banaei Borogeni 2
  • Elham Eftekhari 3
  • Saeed Keshavarz 3
1 PhD candidate, Department of Sports Science, Na.C., Islamic Azad University, NajafAbad, Iran
2 Department of Sports Science, Na.C., Islamic Azad University, NajafAbad, Iran (Corresponding Author)
3 Department of Sports Science, Na.C., Islamic Azad University, NajafAbad, Iran
چکیده [English]

Introduction: The aim of the present study was to investigate the effect of eight weeks of aerobic training (AT) and vitamin E (VE) supplementation on Bax and Bcl-2 in the brain tissue of rats with Parkinson’s disease.
Methods: In this experimental study, 40 male Sprague-Dawley rats (aged 14–16 months, weighing 250–270 g) with Parkinsonism induced by 2 mg/kg of Reserpine (Res) were divided into five groups: (1) Res, (2) Sham (Sh/Vitamin E solvent), (3) AT, (4) VE, and (5) AT+VE. To evaluate the effects of Res on the variables, 8 healthy rats were placed in the healthy control group (HC). Aerobic training was performed for eight weeks, five sessions per week, 15–48 minutes per session at a speed of 10–24 meters per minute. VE was administered orally at a dose of 30 mg/kg per day. One-way ANOVA and Tukey’s post hoc test were used to analyze the data (P ≤ 0.05).
Findings: In the AT, VE, and AT+VE groups, Bax and Bax/Bcl-2 levels were significantly lower and Bcl-2 levels significantly higher than in the Res group (P = 0.001). Moreover, Bax levels in the VE (P = 0.02) and AT+VE (P = 0.001) groups were significantly lower than in the AT group; Bcl-2 levels in the AT+VE group were significantly higher than in the AT and VE groups (P = 0.001)
Conclusion: It seems that both aerobic training and vitamin E, either individually or combined, play a beneficial role in reducing apoptosis markers in brain tissue under Parkinson’s disease conditions.

کلیدواژه‌ها [English]

  • Aerobic Training
  • Vitamin E
  • Apoptosis
  • Parkinson’s Disease

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 68، شماره 2
خرداد-تیر1404
خرداد و تیر 1404
صفحه 416-426

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