نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه ژنتیک مولکولی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران
2 دانشیار، گروه ژنتیک مولکولی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران(نویسنده مسئول)
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Introduction: Polycystic Ovary Syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age, characterized by hormonal imbalances, ovulatory dysfunction, and morphological changes in the ovaries. Understanding the molecular mechanisms of this disease, especially the pathways related to steroidogenesis, is crucial for improving diagnosis and developing targeted therapies.
Methods: This study aimed to investigate the expression patterns of two key steroidogenesis genes—CYP19A1 (encoding the aromatase enzyme) and 17β-HSD (17-beta-hydroxysteroid dehydrogenase)—in a mouse model of PCOS. Thirty-five female NMRI mice were divided into three groups: control (n=5), treated with testosterone enanthate (n=15), and treated with estradiol valerate (n=15). After 25 days of treatment, blood and ovarian samples were collected, and gene expression was assessed using Real-Time PCR. Histological analyses and quantification of ovarian structure were also performed.
Results: The results demonstrated a significant increase in CYP19A1 and 17β-HSD expression in the treated groups (P < 0.0001). While these findings indicate the involvement of these genes in the manifestations of the PCOS mouse model, clinical studies are necessary to validate them as non-invasive biomarkers in humans.
کلیدواژه‌ها [English]
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