نوع مقاله : مقاله پژوهشی
نویسنده
دانشجوی کارشناسی ارشد، گروه ژنتیک مولکولی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسنده [English]
Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine and metabolic disorders in women, characterized by hormonal and metabolic imbalances. MicroRNAs (miRNAs) are recognized as key regulators of gene expression in various biological pathways, including metabolic processes, and play a significant role in the pathophysiology of PCOS.
Objective: This study aimed to evaluate the expression levels of two specific miRNAs, miR-362 and miR-5627, in a mouse model of PCOS to assess their potential roles in the disease and their feasibility as non-invasive biomarkers.
Methods: The PCOS mouse model was induced by treatment with testosterone enanthate and estradiol valerate. Expression levels of miR-362 and miR-5627 were quantified in ovarian tissue and blood samples using quantitative real-time PCR (qRT-PCR) and compared with a control group. Statistical analysis was performed using one-way ANOVA and Tukey’s post hoc tests.
Results: Relative expression of miR-362 and miR-5627 was significantly decreased in both ovarian tissue and blood samples of treated groups compared to controls (P<0.0001). This downregulation indicates a sustained effect of PCOS induction on the regulation of these miRNAs. The similarity of results between blood and ovarian tissue supports the potential use of circulating miRNAs as non-invasive biomarkers.
Conclusion: Significant downregulation of miR-362 and miR-5627 in the PCOS mouse model suggests their involvement in the pathophysiology of PCOS. These miRNAs may serve as molecular biomarkers for diagnosis and monitoring of the disease. The ability to detect these changes in blood samples paves the way for developing non-invasive PCOS monitoring methods. Further functional studies are recommended to explore their therapeutic potential.
کلیدواژهها [English]
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