Expression changes of hemobox-C8 and C9 genes in adipose tissue of obese rats following endurance training and electrical stimulation with calorie restriction

Articles in Press

Document Type : Research Paper

Authors

1 PhD Student in Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, Iran.

2 Assistant Professor, Department of Exercise Physiology, Arak Branch, Islamic Azad University, Arak, Iran.

3 Professor, Department of Exercise Physiology, Tehran north Branch, Islamic Azad University, Tehran, Iran.

10.22038/mjms.2025.83824.4816

Abstract

Introduction
Obesity is an increasing disease and the cause of cardiovascular diseases, high blood pressure, blood fat, diabetes and cancer. Therefore, the aim of the present study was to investigate the changes in the expression of hemobox-C8 and C9 genes in the adipose tissue of obese rats following endurance training and electrical stimulation with calorie restriction.
Material and Method
In this controlled experimental study with a control group of 35 male Wistar rats ( 200±19 g) after induction of obesity and weight gain were randomly divided into 5 groups of 7 without fasting, fasting, fasting-endurance exercise, fasting-electrical stimulation and Fasting-endurance exercise-electrical stimulation were divided. The groups were subjected to endurance training (speed 10 to 20 m/min and duration 20 to 40m), electrical stimulation (foot shock device 0.5 mA and 20 minutes) and fasting (8 to 16 hours) for four weeks. After exercise and anesthesia, adipose tissue was sampled and after performing molecular processes, gene expression was measured using PCR machine.
Results
The results showed that endurance training and its combination with electrical mobility led to a significant increase in hemobox-C8 and a significant decrease in the expression of hemobox-C9 in rats compared to the fasting obese group (P=0.0001 and P=0.001). Also, electrical stimulation showed a significant increase in hemobox-C8 gene (P=0.0001) and a significant decrease in hemobox-C9 gene (P=0.0001).
Conclusion
It seems that endurance training in fasting obese samples with increasing hemobox-8 and decreasing hemobox-9 levels could not cause the conversion of white adipose tissue to brown tissue.

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Medical Journal of Mashhad university of Medical Sciences

Articles in Press, Accepted Manuscript
Available Online from 10 August 2025

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