نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه بیوتکنولوژی، دانشکده مهندسی شیمی، دانشگاه صنعتی انوشیروانی بابل، مازندران، ایران
2 دانشجوی دکترای تغذیه دام، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
3 گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Background and Objectives: Antibacterial resistance has become a global challenge, and the indiscriminate and irrational use of antibiotics in humans and animals has led to the spread of drug resistance in microorganisms. The present study investigated and compared two antibiotics, ciprofloxacin and gentamicin, with rhamnolipid nanoparticles (NPs) coated by chitosan film and gel on two bacteria, Escherichia coli and Staphylococcus aureus.
Methods: In this study, first rhamnolipid was produced from sunflower oil and Pseudomonas aeruginosa and converted into NPS, then 2% chitosan solution was prepared using 1% acetic acid. Then 7.5% glycerin was added as a softener and 0.1% rhamnolipid NPs were added to the obtained homogeneous solution by sampler. Ciprofloxacin and Gentamicin antibiotic discs were used for comparison and antibiogram, Zeta and DLS analysis were used to size rhamnolipid particles.
Results: DLS and ZPS analysis showed that the produced rhamnolipid had a good degree of stability. The antibacterial effects of Gentamicin + chitosan film and rhamnolipid NPs were observed to be the most effective in creating inhibition diameter of 29 mm in Staphylococcus Aureus compared to Escherichia coli, and the effect of Ciprofloxacin + rhamnolipid NPs was also reported in both bacteria with very close inhibition diameters.
Conclusions: The results of this study showed that combining antibiotics with antibacterial materials such as chitosan film and gel with rhamnolipid NPs shows good effects, also new methods such as Nano formulating are a suitable alternative to improve and accelerate therapeutic methods.
کلیدواژه‌ها [English]
)