تاثیر یک دوره تمرین اینتروال با شدت بالا به همراه تحریک الکتریکی با فوت شوک بر فرایند آنژیوژنز بافت کبد موش های صحرایی چاق با محدودیت کالری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی ورزشی، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران.

2 دانشیار، گروه فیزیولوژی ورزشی، دانشگاه آزاد اسلامی‎واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران.

10.22038/mjms.2026.87600.4989

چکیده

مقدمه: چاقی از عوامل اصلی بیماری های کبدی است. این مطالعه بررسی تأثیر ترکیب تمرینات اینتروال شدید (HIIT) و تحریک الکتریکی بر آنژیوژنز کبدی در موشهای چاق تحت محدودیت کالری می باشد.
روش‌ها: این مطالعۀ تجربی با گروه شاهد 35 سر موش صحرایی (8 هفته‌ای) پس از القا چاقی به‌طور تصادفی به 5 گروه7 تایی کنترل، محدودیت کالری ، محدودیت کالری -تمرین تناوبی شدید، محدودیت کالری - تحریک الکتریکی و محدودیت کالری -تمرین تناوبی شدید- تحریک الکتریکی تقسیم شدند. فعالیت هوازی (سرعت 10 -20 متر/دقیقه ، مدت 20 -40 دقیقه)، تحریک الکتریکی ( 5/0 میلی آمپر -20دقیقه) ، محدودیت کالری (8 به 16 ساعت) انجام شد. پس از تمرین ، نمونه‌ برداری بافت کبد صورت گرفت .بیان ژن ها با استفاده از PCR اندازه‌گیری شد. آنالیز داده‌ها با ANOVA سطح معنی‌داری 05/0>p انجام شد.
یافته‌ها: نتایج نشان داد تمرین تناوبی شدید در گروه چاق، تحت محدودیت کالری باعث افزایش معنی‌دار بیان ژن VEGFنسبت به گروه چاق گردید (0001/0>p). HIIT باعث افزایش معنی دار FGF23 در این گروه نسبت به گروه چاق نشد ( 0001/0 P >). تلفیقHIIT با تحریک الکتریکی در گروه چاق با محدودیت کالری باعث افزایش معنی‌دار بیان ژن VEGF و کاهش معنی‌دار بیان ژن FGF23 نسبت به گروه چاق شد. (0001/0>p)
نتیجه‌گیری: به نظرمی رسد، HIIT و تحریک الکتریکی حین محدودیت کالری می تواند باعث افزایش معنی‌دار ژن VEGF و کاهش معنی دار ژن FGF23 گروه چاق با محدودیت کالری شود و در سلامت کبد و افزایش آنژیوژنز موثر باشد. ‌اما ابعاد آن نیازمند مطالعات بیشتری است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of high-intensity interval training along with electrical stimulation using foot shock on the process of angiogenesis in the liver tissue of obese rats to calorie restriction

نویسندگان [English]

  • Farkhunde Yarahmadi 1
  • Mohammad Malekipooya 2
  • Sara Hamedi 1
1 Department of Exercise Physiology, Ar.C., Islamic Azad University, Arak, Iran
2 Associate Professor, Department of Exercise Physiology, Ar.C., Islamic Azad University, Arak, Iran.
چکیده [English]

Background: Obesity is one of the main factors of liver diseases. This study examines the effect of combining high-intensity interval training (HIIT) and electrical stimulation on liver angiogenesis in obese rats under caloric restriction.
Methods: This experimental study with a control group included 35 (eight-week-old) rats, which, after inducing obesity, were randomly divided into five groups of seven: control, caloric restriction, caloric restriction–high-intensity interval training (HIIT), caloric restriction–electrical stimulation, and caloric restriction–HIIT–electrical stimulation. Aerobic activity (speed: 10–20 meters per minute, duration: 20–40 minutes), electrical stimulation (0.5 milliampere, 20 minutes), and caloric restriction (8–16 hours) were implemented. After the interventions, liver tissue sampling was performed. Gene expression was measured using PCR, and data analysis was conducted using ANOVA with a significance level of p<0.05.
Results: The results showed that high-intensity interval training in the obese group under caloric restriction caused a significant increase in VEGF gene expression compared to the obese group (p < 0.0001). HIIT did not cause a significant increase in FGF23 in this group compared to the obese group (p > 0.0001). The combination of HIIT with electrical stimulation in the obese group with caloric restriction caused a significant increase in VEGF gene expression and a significant decrease in FGF23 gene expression compared to the obese group (p < 0.0001)."
Conclusion: HIIT and electrical stimulation during caloric restriction may significantly increase VEGF and decrease FGF23 in obese rats, improving liver health and angiogenesis. However, further research is needed to explore its broader implications

کلیدواژه‌ها [English]

  • Obesity
  • Caloric restriction
  • High-Intensity Interval Training
  • Electrical Stimulation
  • Angiogenesis

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 69، شماره 4
تیر 1405
تیر 1405
صفحه 476-491

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