1-Dranser K. Local Anesthetics. In: Katzung BG. Vanderah TW. Basic and Clinical Pharmacology. 15 th
ed. MC Graw Hill. 2021
2-Murphy J T, Allen H F, Mangiaracine AB, (1955) Preparation, sterilization, and preservation of
ophthalmic solutions¨experimental studies and a practical method. Arch Ophthalmol 53: 63-78.
3-Conte BA, Laforet EG (1962) The role of the topicall anaesthetics agents in modifying bacteriological
data obtained by bronchoscopy. New Eng J Med 267:957-960.
4-Silva MT, Sousa JCF, Polonia JJ, Macedo PM (1979) Effect of local anaesthetics on bacterial cells. J
Bact 137:461-468.
5. Johnson SM, Saint John BE, Dine AP (2008) Local anesthetics as antimicrobial agents: a review. Surg
Infect 9:205–213.
6-Razavi M, Fazly Bazzaz BS (2019) A review and new insights to antimicrobial action of local
anesthetics, Eur J Clin Microbiol Infect Dis. 38:991-1002.
7- Park KK, Sharon VR (2017) A review of local anesthetics: minimizing risk and side effects in
cutaneous surgery. Dermatol Surg 43:173–187.
8- Jung RM, Rybak MA, Milner PT et al (2017) Local anesthetics and advances in their administration:
an overview. J Pre-Clin Clin Res 11:94–101.
9- Keld DB, Hein L, Dalagaard M, Krogh L, Rodt SA (2000) The incidence of transient neurologic
symptoms (TNS). Acta Anaesthsiol Scand. 44: 285-290.
10-Ghaemi M, Ansari M, Mohammadi S (2006) Comparison of the sensory-motor block effects of low
dose hyperbaric bupivacaine and lidocaine in anorectal surgery with spinal anesthesia, J Advance Med
Biomed Res 14:1-5.
11-Adler DMT Damborg P, Verwilghen DR (2017) The antimicrobial activity of bupivacaine, lidocaine
and mepivacaine against equine pathogens: An investigation of 40 bacterial isolates, The Veterinary
Journal. 223: 27-31.
12-45. Rota S, Akcabay M, Emektas G et al (1993) Antibacterial activity of bupivacaine. Gazi Tip
Dergisi 4:69–71.
13. Pere P, Lindgren L, Vaara M (1999) Poor antibacterial effect of ropivacaine: comparison with
bupivacaine. Anesthesiology 91:884–886.
14. Grimmond TR, Brownridge P (1986) Antimicrobial activity of bupivacaine and pethidine. Anaesth
Intensive Care 14:418–420.
15- Rosenberg PH, Renkonen OV (1985) Antimicrobial activity of bupivacaine and morphine.
Anesthesiology 62:178–179.
16- Sakuragi T, Ishino H, Dan K (1998) Bactericidal activity of preservative-free bupivacaine on
microorganisms in the human skin flora, Acta Anaesthesiol Scand. 42:1096-9.
17- Sakuragi T, Ishino H, Dan K (1997) Bactericidal activity of 0.5% bupivacaine with preservatives on
microorganisms in the human skin flora. Reg Anesth 22:178–184.
18- Aydin ON, Eyigor M, Aydin N (2001) Antimicrobial activity of ropivacaine and other local
anaesthetics. Eur J Anaesthesiol 18:687–694.
19- Hodson M, Gajraj R, Scott NB (1999) A comparison of the antibacterial activity of levobupivacaine
vs. bupivacaine: an in vitro study with bacteria implicated in epidural infection. Anaesthesia 54:699–702.
21- Morrow ME, Berry CW (1988) Antimicrobial properties of topical anesthetic liquids containing
lidocaine or benzocaine. Anesth Prog 35:9–13.
22- Lagan G, McLure H (2004) Review of local anaesthetic agents. Curr Anaesth Crit Care 15:247–254.
23-Kerenyi M, Batai R, Juhasz V et al (2004) Lidocaine/prilocaine cream (EMLA) has antibacterial effect
in vitro. J Hosp Infect 55:75–76.
24-Batai I, Bogar L, Juhasz V et al (2009) A comparison of the antimicrobial property of
lidocaine/prilocaine cream (EMLA®) and an alcohol-based disinfectant on intact human skin flora.
Anesth Analg 108:666–668.
25- Berg JO, Mössner BK, Skov MN et al (2006) Antibacterial properties of EMLA® and lidocaine in
wound tissue biopsies for culturing. Wound Repair Regen 14:581–525.
26-Meyler's, (2016) Side Effects of Drugs (Sixteenth Edition).
27- Fazly Bazaz BS, Salt WG (1983) Local anaesthetics as antimicrobial agents: structure-action
considerations. Microbios 37:45–64.
28-Fazly Bazaz BS, Salt WG (1983) Local anaesthetics as antibacterial agents: effects on cellular
respiration and the leakage of cytoplasmic constituents. Microbios 37:139–149.
29-Fazly Bazaz BS, Salt W (1983) Local anaesthetics induced turbidity increases: implications of
interactions with intact bacterial cells and with subcellular fractions. Microbios 36:135–147.
30-Beckett A, Patki SJ, Robinson AE (1959) The interaction of phenolic compoundswith bacteria, ll. The
effect of various substances on the interactionof hexylresorcinol with Escherichia coli, J Pharm
Pharmacol. 11:367-373
31-Hugo WB, Longwort AR (1964) some aspects of the mode of action of chlohexidine. J Pharm
Pharmacol. 16:655-662.
32- Batai I, Kerenyi M, Falvai J et al (2002) Bacterial growth in ropivacaine hydrochloride. Anesth Analg
94:729–731.
33- Gordh T, Gordh TE, Lindqvist K, Warner DS (2010) Lidocaine: The origin of a modern local
anesthetic, Anesthesiology. 113:1433–1437.
34-Neuwersch S, Kostenberger M, Sorschag S, Ilias W, Likar R (2017) Antimicrobial activity of
lidocaine, Bupivacaine, mepivacaine and ropivacaine on Staphylococcus epidermidis, Staphylococcus
aureus and Bacillus subtilis, The Open Pain Journal.10:1-4.
35- Kim S, Toma H, Midha N et al (2010) Antibiotic resistance of conjunctiva and nasopharynx
evaluation study: a prospective study of patients undergoing intravitreal injection. Ophthalmology
117:2372–2378.
36- Tustin A, Kim SJ, Chomsky A et al (2014) Antibacterial propertiesof 2% lidocaine and reduced rate
of endophthalmitis after intravitreal injection. Retina 34:935–942.
37- Tabacov I, Tsatsova V (1970) The antibacterial effect of a chlorhexidine-lidocaine jelly upon the flora
of the anterior male urethra. Int Urol Nephrol 2:199–202.
38- Yang H, Guo X, Chen X et al (2013) Study on antibacterial effect of lidocaine. Chinese J Androl
27:14–16.
39- Srisatjaluk RL, Klongnoi B, Wongsirichat N (2016) Antimicrobial effect of topical local anesthetic
spray on oral microflora. J Dent Anesth Pain Med 16:17–24.
40- Aldous WK, Jensen R, Sieck BM (1998) Cocaine and lidocaine with phenylephrine as topical
anesthetics: antimicrobial activity against common nasal pathogens. Ear Nose Throat J 77:554–557.
41- Chandan SS, Faoagali J, Wainwright CE (2005) Sensitivity of respiratory bacteria to lignocaine.
Pathology 37:305–307.
42- Feldman JM, Chapin-RRoberison K, Tumer J (1994) Do agents used for epidural analgesia have
antimicrobial properties? Reg Anesth 19:43-47.
43- Begec Z, Gulhas N, Toprak HI et al (2007) Comparison of the antibacterial activity of lidocaine 1%
versus alkalinized lidocaine in vitro. Curr Ther Res Clin Exp 68:242–248.
44- Pina-Vaz C, Rodrigues AG, Sansonetty F et al (2000) Antifungal activity of local anesthetics against
Candida species. Infect Dis Obstet Gynecol 8:124–137.
45- Rodrigues A, Pina-Vaz C, Mardh P et al (2000) Inhibition of germ tube formation by Candida
albicans by local anesthetics: an effect related to ionic channel blockade. Curr Microbiol 40:145–148.
46- Rodrigues AG, Araujo R, Pina-Vaz C (2006) Interaction of local anaesthetics with other antifungal
agents against pathogenic aspergillus. Int J Antimicrob Agents 27:339–343.
47- Sakuragi T, Ishino H, Dan K (1996) Bactericidal activity of clinically used local anesthetics on
Staphylococcus aureus. Reg Anesth 21:239–242
48- Pal Sh, Sayana A, Joshi A, Juyal D (2019) Staphylococcus aureus: A predominant cause of surgical
site infections in a rural healthcare setup of Uttarakhand, J Family Med Prim Care. 8: 3600–3606.
49-Filsoufi F, Castillo J, Rahmanian P et al (2009) Epidemiology of deep sternal wound infection in
cardiac surgery. J Cardiothorac Vasc Anesth 23:488–494.
50- Parr AM, Zoutman DE, Davidson JSD (1999) Antimicrobial activity of lidocaine against bacteria
associated with nosocomial wound infection. Ann Plast Surg 43:239–245.
51- Lu CW, Lin TY, Shieh JS et al (2014) Antimicrobial effect of continuous lidocaine infusion in a
Staphylococcus aureus-induced wound infection in a mouse model. Ann Plast Surg 73:598–601.
52-Allison C Scully, James R Boynton (2017) Antibiotics and Local Anesthetics: Drug Considerations
for the Child Patient, J Mich Dent Assoc 99:36-41, 71.
53- Rodrigues AG, Araujo R, Pina-Vaz C (2006) Interaction of local anaesthetics with other antifungal
agents against pathogenic aspergillus. Int J Antimicrob Agents 27:339–343.
54- Ton That V, Nguyen S, Poon D et al (2010) Bioluminescent lux gene biosensors in oral streptococci:
determination of complementary antimicrobial activity of minocycline hydrochloride with the anesthetic
lidocaine/prilocaine or the antiseptic chlorhexidine. InPeriodontitis: symptoms, treatment and prevention.
Nova Science Publishers, Inc, p 141–165.
55- Barza M, Ernst C, Baum J et al (1974) Effect of lidocaine on the antibacterial activity of seven
antibiotics. Arch Ophthalmol 92:514–515.
56- Pöpping DM , Elia N, Marret E, Wenk M, Tramèr MR (2012) Opioids added to local anesthetics
for single-shot intrathecal anesthesia in patients undergoing minor surgery: a meta analysis of randomized
trials, Pain 153:784-793.
57-Sevgi Kesici, Mehmet Demirci, Ugur Kesici (2020) Antimicrobial effects of fentanyl and bupivacaine:
an in vitro study, Brazilian Journal of Anesthesiology 70:357-363.
58- Pirbudak L, Karsligil T, Zer Y et al (2005) Antibacterial effect of bupivacaine and ropivacaine; effect
of adjuvant drugs. Pain Clin 17:73–80.
59- Feldman JM, Chapin-Robertson K, Turner J (1994) Do agents used for epidural analgesia have
antimicrobial properties? Reg Anesth 19:43–47.
60- Kampe S, Poetter C, Buzello S et al (2003) Ropivacaine 0.1% with sufentanil 1 microg/mL inhibits in
vitro growth of Pseudomonas aeruginosa and does not promote multiplication of Staphylococcus aureus.
Anesth Analg 97:409–411.
61- Guillier M, Boselli E, Bouvet L et al (2007) Levobupivacaine hydrochloride and sufentanil have no
antimicrobial effect at 25°C in vitro. Eur J Anaesthesiol 24:634–639.
62-Tamanai-Shacoori Z, Shacoori V, Vo Van JM et al (2004) Sufentanil modifies the antibacterial
activity of bupivacaine and ropivacaine. Can J Anesth 51:911–914.
63- Yagiela JA (1995) Vasoconstrictor agents for local anesthesia. Anesth Prog 42:116–120.
64-Ugur Kesici, Mehmet Demirci, Sevgi Kesici (2019) Antimicrobial effects of local anaesthetics, Int
Wound 16:1029–1033.
65- Won Keun S, Hyang Joon P, You Chan K et al (2000) The antimicrobial effects of lidocaine and
epinephrine. Korean J Dermatol 38:635–642.
66- Stratford AF, Zoutman DE, Davidson JS (2002) Effect of lidocaine and epinephrine on
Staphylococcus aureus in a guinea pig model of surgical wound infection. Plast Reconstr Surg 110:1275–
1279.
67- Sculley PD, Dunley RE (1980) Antimicrobial activity of a lidocaine preparation. Anesth Prog 27:21–
23.
68- Gajraj RJ, Hodson MJ, Gillespie JA et al (1998) Antibacterial activity of lidocaine in mixtures with
Diprivan. Br J Anaesth 81:444–448.
69- Euasobhon P, Dej-Arkom S, Siriussawakul A et al (2016) Lidocaine for reducing propofol-induced
pain on induction of anaesthesia in adults. Cochrane Database Syst Rev 2: Cd007874
70- Sakuragi T, Yanagisawa K, Shirai Y et al (1999) Growth of Escherichia coli in propofol, lidocaine,
and mixtures of propofol and lidocaine. Acta Anaesthesiol Scand 43:476–479.
71- Wachowski I, Jolly DT, Hrazdil J et al (1999) The growth of microorganisms in propofol and
mixtures of propofol and lidocaine. Anesth Analg 88:209–212.
72- Driver R, Granus V, Yassa Y (1998) Growth inhibition of Staphylococcus aureus by
propofol/lidocaine admixture. Anesth Analg 86:166S.
73- Curatolo M, Petersen-Felix S, Arendt-Nielsen L et al (1998) Adding sodium bicarbonate to lidocaine
enhances the depth of epidural blockade. Anesth Analg 86:341–347.
74- Snyders SP, James MFM (2008) Alkalinisation does not enhance the antimicrobial properties of local
anaesthetic solutions. S Afr J Anaesth Analg 14:25–28.
75- Thompson KD, Welykyj S, Massa MC (1993) Antibacterial activity of lidocaine in combination with
a bicarbonate buffer. J Dermatol Surg Oncol 19:216–220.
76- Begeç Z, Gülhaş N, Toprak HI et al (2007) Antibacterial effectiveness of alkalinized ropivacaine and
bupivacaine. Turk Anesteziyoloji ve Reanimasyon Dernegi Dergisi 35:11–15.
77- Pelz K, Wiedmann-Al-Ahmad M, Bogdan C et al (2008) Analysis of the antimicrobial activity of
local anaesthetics used for dental analgesia. J Med Microbiol 57:88–94.
78- Wimberley N, Willey S, Sullivan N et al (1979) Antibacterial properties of lidocaine. Chest 76:37–40.
79- Schmidt RM, Rosenkranz HS (1970) Antimicrobial activity of local anesthetics: lidocaine and
procaine. J Infect Dis 121:597–607.
80- Ohsuka S, Ohta M, Masuda K et al (1994) Lidocaine hydrochloride and acetylsalicylate kill bacteria
by disrupting the bacterial membrane potential in different ways. Microbiol Immunol 38:429–434.
81- Leung Y, Rawal B (1977) Mechanism of action of tetracaine hydrochloride against Pseudomonas
aeruginosa. Infect Dis Obstet Gynecol 136:679–683.
82- Salt W, Wiseman D (1970) The effect of magnesium ions and Tris-buffer on the uptake of cetyl
trimethyl ammonium bromide by Escherichia coli. J Pharm Pharmacol 22:767–773.
83- Lamikanara A, Allwood M (1977) Effect of polyoxyalkylphenols on the optical density of
Staphylococcus aureus. J Appl Bacteriol 42:387–392.
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