مروری بر روش‌های فنوتیپی تشخیص مقاومت آنتی بیوتیکی ناشی از آنزیم کارباپنماز در باکتری‌های جدا شده از نمونه های بالینی

نوع مقاله : مقاله مروری

نویسندگان

1 گروه آموزشی میکروب شناسی وایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی کاشان، کاشان، ایران

2 گروه میکروبیولوژی، انستیتو پاستور ایران، تهران، ایران

3 دانشکده داروسازی، دانشگاه آزاد اسلامی واحد تهران

4 دانشکده پزشکی، دانشگاه آزاد اسلامی واحد قم

5 گروه میکروب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی تهران، تهران، ایران

چکیده

در سال های اخیر، عفونت‌های ناشی از باکتریهای گرم منفی مولد آنزیم‌های کارباپنماز به یکی از چالش‌ها و نگرانی‌های بهداشت جهانی تبدیل شده است. شناسایی سریع و دقیق باکتری‌های گرم منفی مولد آنزیم کارباپنماز می‌تواند در کنترل بیماری‌های عفونی و درمان به موقع آنها موثر واقع شود. طی سال‌های گذشته، تعداد فزاینده‌ای از روش‌های فنوتیپی برای تشخیص سریع فعالیت کارباپنماز باکتری‌های گرم منفی توسعه یافته است. با این حال، هیچ روش فنوتیپی واحدی برای شناسایی آنزیم‌های کارباپنماز که دارای حساسیت و اختصاصیت 100% و حاوی همه ویژگی‌های آزمایش ایده آل باشد، وجود ندارد. انتخاب رویکرد انجام آزمایش مناسب به عوامل مختلفی نظیر وضعیت اپیدمیولوژیک، منابع آزمایشگاهی و در دسترس بودن سایر آزمایش های تأییدی بستگی دارد. مطابق با انچه در این تحقیق بررسی شده است، تست های کاربرپسند، مقرون به صرفه، دقیق و قابل اجرا در آزمایشگاه‌های میکروب شناسی بالینی وجود دارد. در این مقاله مروری، روش‌های فنوتیپی مختلف موجود برای تشخیص فعالیت کارباپنماز در باکتری‌های گرم منفی مورد ارزیابی قرار گرفته است.

کلیدواژه‌ها

عنوان مقاله [English]

A review of phenotypic methods for detecting antibiotic resistance induced by carbapenemase enzyme in bacteria isolated from clinical specimens

نویسندگان [English]

  • Mehrdad Mohammadi 1
  • Masoumeh Beig 2
  • Kimia Barikrou 3
  • Sima Soltani 4
  • Leila Ali Nasab Maleki 4
  • Parsa Veisi 4
  • Samane Teymouri 5
1 Department of Microbiology and immunology, school of Medicine,, Kashan university of medical sciences, Kashan ,Iran
2 Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
3 Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran,Iran
4 Department of Medicine, Qom Branch, Islamic Azad University, Qom, Iran
5 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
چکیده [English]

In recent years, infections caused by gram-negative organisms that produce carbapenemase enzymes (CPO) have become one of the global health challenges and concerns. Rapid and accurate identification of gram-negative organisms that produce the carbapenemase enzyme can be effective in controlling infectious diseases and their timely treatment. Over the years, an increasing number of phenotypic methods have been developed to rapidly detect the activity of gram-negative carbapenemases. However, there is no single phenotypic method that has 100% sensitivity and specificity and contains all the characteristics of an ideal test for the detection of carbapenemase enzymes. The choice of an appropriate methodological approach depends on various factors such as epidemiological status, laboratory resources, and the availability of other confirmatory tests. According to what has been studied in this research, there are user-friendly, cost-effective, accurate, and applicable tests in clinical microbiology laboratories. In this review study, various phenotypic methods have been evaluated for detecting carbapenemase activity in gram-negative bacteria.

کلیدواژه‌ها [English]

  • Carbapenemase
  • Enterobacteriaceae
  • Beta-lactam
  • antibiotic resistance

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 65، شماره 1
فروردین و اردیبهشت 1401
صفحه 148-171

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