تاثیر تمرین تناوبی و عسل آویشن بر مسیر miR-423-5p، FAM3A، AKt2 کبدی موش‌های صحرایی دیابتی نوع دو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دبیر آموزش و پرورش ناحیه یک شیراز

2 هیات علمی دانشگاه آزاد اسلامی واحد تهران جنوب، دانشکده تربیت بدنی و علوم ورزشی، گروه فیزیولوژی ورزش، دانشیار

3 هیات علمی دانشگاه آزاد اسلامی واحد تهران جنوب، دانشکده تربیت بدنی و علوم ورزشی، گروه فیزیولوژی ورزشی، استادیار

4 گروه تربیت بدنی و علوم ورزشی، دانشگاه ازاد اسلامی واحد علوم تحقیقات تهران، تهران، ایران.

چکیده

مقدمه: میکروRNAها در ایجاد دیابت نوع۲ و بروز عوارض آن دخالت دارند. هدف پژوهش حاضر مطالعه تاثیر تمرین تناوبی و عسل آویشن بر چگونگی بیان ژن های مسیر miR-423-5P- FAM3A-AKt2 در بافت کبدی موش‌های صحرایی دیابتی نوع۲ بود.
روش‌ کار: ۳۶ سرموش نرویستار پس از۲۰هفته تغذیه با رژیم غذایی پرچرب و سپس تزریق استرپتوزتوسین به میزان ۲۵میلی‌گرم/کیلوگرم برای القا دیابت نوع۲، به چهارگروه دیابتی با کبد چرب: کنترل، تمرین تناوبی، عسل آویشن و تمرین همراه با عسل تقسیم شدند. هشت هفته تمرین تناوبی، پنج جلسه در هفته برای گروه های تمرینی انجام و عسل آویشن روزانه با دُز ۳ گرم/کیلوگرم به روش گاواژ به موش‌های گروه مداخله تغذیه ای خورانده شد. میزان بیان ژن هایmiR-423-5P، mRNA FAM3Aو mRNA AKt2 کبد با استفاده از روش RT-PCR و بکارگیری کیت های مخصوص اندازه گیری و یافته ها با استفاده از آزمون تحلیل واریانس دو عاملی و آزمون تعقیبی بونفرونی مورد تجزیه و تحلیل قرار گرفت.
نتایج: در گروه های تجربی نسبت به گروه کنترل بیان ژن miR-423-5P کاهش معنی دار و بیان mRNA FAM3A افزایش غیرمعنی دار داشت و بیان mRNA AKt2 در گروه های تمرین و تمرین همراه با عسل نسبت به گروه کنترل افزایش معنی دار و در گروه عسل نیز افزایش داشت که از نظر آماری معنی دار نبود.( 0/05>P)
نتیجه‌گیری: به نظر می‌رسد تمرین تناوبی و مصرف عسل آویشن هر دو به تنهایی وبه صورت تعاملی با تاثیر بیشتر، بتوانند به ایجاد تغییرات مطلوب در بیان ژن های مذکور در کبد موش‌های صحرایی دیابتی نوع ۲ کمک کنند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of High Intensity Interval Training and Thyme Honey on Hepatic miR-423-5P, FAM3A, Akt2 Pathway of Type ll Diabetic rats

نویسندگان [English]

  • marjan abdi ardekani 1
  • Abdol Ali Banaei Far 2
  • Sajjad Arshadi 3
  • Hossein Abednatanzi 4
1 Teacher of district one of education in Shiraz
2 Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, Islamic Azad University, South Tehran Branch, Tehran. Iran.
3 Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, Islamic Azad University, South Tehran Branch, Tehran. Iran.
4 Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
چکیده [English]

Introduction: Micro-RNAs are effective in inflicting diabetes type II and its side effects. The Purpose of this study was to evaluate the effect of High Intensity Interval Training & thyme honey on expression of the genes of the hepatic miR-423-5P - FAM3A-AKt2 pathway in rats.
Materials and Methods: 36 male rats were fed with high-fat diets for 20weeks and were then injected streptozotocin (25 mg/kg) in order to inflict diabetes type II. The 36rats were then divided into four groups: Control, regular exercise group, nutritional intervention group and exercise with nutritional intervention group. Regular exercise included five sessions a week for eight weeks and nutritional intervention was done at a dosage of 3 g/kg of thyme honey using a gauge method. The amount of expression of miR-423-5P, mRNA FAM3A and mRNA AKt2 genes of the liver were assessed using RT-PCR and evaluation kits. The obtained findings were analyzed using the two-way analysis of variance and Bonferroni post hoc test.
Results: Expression of the miR-423-5P gene was significantly lower in all groups compared to control, expression of mRNA FAM3 had insignificant increase and expression of mRNA AKt2 had a significant increase in groups of regular exercise and exercise with nutritional intervention. This gene had an insignificant increase of expression in the group with only nutritional intervention. (p value<0.05)
Discussion: It seems that HIIT & consumption of thyme honey, both alone and interactively with greater effectiveness, can change in expression of the mentioned gene in liver of rats with diabetes type II.

کلیدواژه‌ها [English]

  • Diabetes Type II
  • miR-423-5P - FAM3A -AKt2
  • HIIT
  • Thyme Honey

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 65، شماره 1
فروردین و اردیبهشت 1401
صفحه 101-115

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