تاثیر پنج هفته تمرین تناوبی شدید بر بیان ژن‌های RXFP1 و CTGF بافت قلبی و آنزیم کبدی SGPT در رت‌های ویستار نر مبتلا به کبد چرب

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش،گروه تربیت بدنی، واحد بروجرد،دانشگاه آزاد اسلامی،بروجرد،ایران

2 استادیار فیزیولوژی ورزش، گروه تربیت بدنی، واحد بروجرد،دانشگاه آزاد اسلامی،بروجرد،ایران

3 دانشیار فیزیولوژی ورزش،مرکز تحقیقات فیزیولوژی ورزشی،پژوهشکده سبک زندگی،دانشگاه علوم پزشکی بقیهّ الله(عج)،تهران،ایران

چکیده

زمینه و هدف: در سالهای اخیر مطالعاتی درموردارتباط کبدچرب و آتروسکلروزیس انجام شده است که نتایج این مطالعات، حاکی از ارتباط کبد چرب غیرالکلی با آترواسکلروزیس عروق کرونری می باشد. کبد چرب با افزایش خطر بیماریهای قلبی- عروقی همراهی دارد. افزایش در ضخامت لایه اینتمیا مدیا شریان کاروتید و تنگی شریان کاروتید مشترک ثانویه به پلاکهای کاروتید، نشانگرهای آترواسکلروزیس هستند. هدف از این مطالعه اثر تمرین تناوبی شدید (HIIT) بر بیان ژن‌های قلبی1 RXFP و CTGF رت های نر مبتلا به کبد چرب مدل استئاتوزیس بود.
مواد و روشها: 48 سر رت نر ویستار با وزن 230 – 300 گرم در 6 گروه پایه سالم (BH) ،گروه پایه مدل شده (استئاتوزیس)(BS) ، گروه HIIT سالم(HIIT) ،گروه HIIT مدل شده (استئاتوزیس)(SHIIT)، گروه کنترل سالم(CH)، گروه کنترل مدل شده(استئاتوزیس)(CS) به صورت تصادفی تقسیم شدند. موشهای گروه کبد چرب به مدت دو هفته، روزانه، تتراسایکلین خوراکی دریافت کردند. رت های گروه های تمرینی نیز به مدت 5 هفته/ پنح روز تمرین داده شدند .دو گروه BS و BH در پایان هفته دوم قربانی شدند و گروههای تمرینی و گروه CS در پایان هفته 5 قربانی شده و نمونه بافت قلب جهت بررسی بیان ژن های SGPT، RXFP1 و CTGF گرفته شد.
یافته ها: میزان SGPT در گروههای BS و CS بطور معناداری نسبت به 4 گروه دیگر بالاتر بود. سطح این آنزیم در گروههای HIIT و SHIIT نسبت به گروههای کبد چرب کاهش معناداری داشت. میزان بیان ژن RXFP1 در گروههای BS,CS و SHIIT بطور معناداری نسبت به 3 گروه دیگر بالاتر بود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of five weeks of intense intermittent training on the expression of RXFP1 and CTGF genes in heart tissue and liver enzyme SGPT in male Wistar rats with fatty liver.

نویسندگان [English]

  • Masoud Hamzeh lou 1
  • Mania Roozbayani 2
  • Hosein Shirvani 3
1 Phd student of exercise physiology, Department of Physical Education, Borujerd branch, Islamic Azad University, Borujerd, Iran
2 1- Assistant Professor of Exercise Physiology, Department of Physical Education, Borujard Branch, Islamic Azad University, Borujard, Iran
3 Associate Professor of Sports Physiology, Sports Physiology Research Center, Lifestyle Research School, Baqiyatullah University of Medical Sciences, Tehran, Iran
چکیده [English]

Background and purpose: In recent years, studies have been conducted on the relationship between fatty liver and atherosclerosis, and the results of these studies indicate the relationship between non-alcoholic fatty liver and atherosclerosis of coronary arteries. Fatty liver is associated with increased risk of cardiovascular diseases. An increase in the thickness of the intima-media layer of the carotid artery and stenosis of the common carotid artery secondary to carotid plaques are indicators of atherosclerosis. The aim of this study was the effect of high intensity interval training (HIIT) on the expression of cardiac 1 RXFP and CTGF genes in male rats with fatty liver steatosis model.
Materials and methods: 48 male Wistar rats weighing 230-300 g in 6 basic healthy groups (BH), modeled basic group (steatosis) (BS), healthy HIIT group (HIIT), modeled HIIT group (steatosis) SHIIT), healthy control group (CH), modeled control group (steatosis) (CS) were randomly divided. Rats in the fatty liver group received oral tetracycline daily for two weeks. The rats of the training groups were also trained for 5 weeks/5 days. Two groups, BS and BH, were sacrificed at the end of the second week, and the training groups and the CS group were sacrificed at the end of the 5th week, and heart tissue samples were taken to examine the expression of SGPT genes. , RXFP1 and CTGF were taken.
Findings: The amount of SGPT in BS and CS groups was significantly higher than the other 4 groups.

کلیدواژه‌ها [English]

  • Intermittent intense training
  • RXFP1
  • CTGF
  • fatty liver

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