اثر تمرین تناوبی و مصرف مکمل نانو سلنیوم بر بیان ژن‎های HSP70 و PGC-1α بیضه در رت‎های تحت القای دگزامتازون

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد تهران شرق، دانشگاه آزاد اسلامی‎تهران، ایران

2 استادیارفیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد تهران شرق، دانشگاه آزاد اسلامی‎ تهران، ایران(نویسنده مسئول)

3 استاد فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد محلات، دانشگاه آزاد اسلامی، محلات، ایران

چکیده

مقدمه: تمرین ورزشی و سلنیوم راهکاری مؤثر برای تقویت سیستم ایمنی است. هدف از این پژوهش تأثیر تمرین تناوبی و  مصرف مکمل نانو سلنیوم بر بیان ژن‎های HSP70 و PGC-1α بیضه در رت‎های تحت القای دگزامتازون است.
روش‌کار: 40 سر رت نژاد ویستار در محدوده سنی 10 هفته که به طور تصادفی در 5 گروه 8 نفره (سالم، تحت القای دگزامتازون (بیمار)، بیمار+تمرین تناوبی طولانی مدت، بیمار+ مصرف مکمل نانوسلنیوم، بیمار+تمرین تناوبی طولانی مدت+ مصرف مکمل نانوسلنیوم) تقسیم شدند. برنامه تمرینی شامل 12-5 تکرار 1 دقیقه‎ای دویدن روی نوارگردان جوندگان با شدت 24 تا 30 متر در دقیقه، معادل 75 تا 100 درصد بیشینه اکسیژن مصرفی، با فواصل استراحت فعال 75 ثانیه‎ای بود. این تمرینات شش روز در هفته به مدت چهار هفته اجرا شد. محلول نانوذرات سلنیوم آماده شده، به میزان 100 میلی‌گرم در سایز 250 نانومتر به صورت گاواژ و یک روز درمیان به موش‎ها‎ داده شد. از تحلیل واریانس سه راهه برای تعیین اختلاف در متغیرهای بین گروهها در سطح معناداری (05/0> P) استفاده شد. کلیه محاسبات آماری با استفاده از نرم افزار Excel و SPSS نسخه 25 انجام گرفت.
نتایج: نتایج به‌دست‌آمده از پژوهش نشان داد تمرین تناوبی منجر به افزایش معنادار HSP70 شد(05/0>P ). همچنین بیان ژن  PGC-1α در گروه مکمل  نانو سلنیوم و تمرین تناوبی منجر به افزایش معنا داری شد (05/0>P ).
نتیجه­گیری: با توجه به پژوهش حاضر به نظر می‎رسد تمرین تناوبی و مصرف مکمل نانوسلنیوم منجر به افزایش معنادار HSP70 و PGC-1α می‎شود که نقش مهمی ‎در بهبود عملکرد ایمنی، بیماران دچار ضعف سیستم ایمنی دارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of interval training and Nano selenium supplementation on the expression of testicular HSP70 and PGC-1α genes in dexamethasone induced rats

نویسندگان [English]

  • Omid Soleimani Ghaleh 1
  • Shahin Riyahi Malayeri 2
  • Bahram Abedi 3
1 MSc in Exercise Physiology, Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.
2 Assistant Professor of Exercise Physiology, Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.(Corresponding Author)
3 Professor of Exercise Physiology, Department of Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, Iran.
چکیده [English]

Introduction: Exercise Training and selenium is an effective way to strengthen the immune system. The purpose of this research is the effect of interval training and nano selenium supplementation on the expression of testicular HSP70 and PGC-1α genes in dexamethasone induced rats.
Materials and Methods: 40 Wistar rats aged 10 weeks were randomly divided into 5 groups of 8 (healthy, under dexamethasone induction (patient), patient + interval training, patient + Nano selenium supplement, patient + interval training + consumption Nano selenium supplement) were divided. The exercise program consisted of 5-12 repetitions of 1-minute running on the rodent treadmill with an intensity of 24-30 m/min, equivalent to 75-100% of maximal oxygen consumption, with active rest intervals of 75 seconds. These exercises were performed six days a week for four weeks. The prepared selenium nanoparticle solution, 100 mg in 250 nm size, was given to mice by gavage every other day. Three-way analysis of variance was used to determine the difference in variables between groups at a significant level (P<0.05). All statistical calculations were done using Excel and SPSS version 25 software.
Results: The results obtained from the research showed that interval training led to a significant increase in HSP70 (P<0.05). Also, the expression of PGC-1α gene in the group of Nano selenium supplementation and interval training led to a significant increase (P<0.05).
Conclusion: According to the present study, it seems that interval training and Nano selenium supplementation leads to a significant increase in HSP70 and PGC-1α, which plays an important role in improving the immune function of patients with weak immune system.

کلیدواژه‌ها [English]

  • Interval training
  • Nano selenium supplement
  • HSP70
  • PGC-1α
  • dexamethasone

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 65، شماره 2
خرداد و تیر 1401
صفحه 645-653

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