تأثیر توام گرسنگی و تمرین هوازی بر شاخص‌های خطر زای، توده بدن و بیان ژن ATF6 شبکه آندوپلاسمی‎ کبد سالم رت‌های نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران

2 استادیار تربیت بدنی و علوم ورزشی، گروه تربیت بدنی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران

3 استادیار فیزیولوژی ورزشی ،گروه تربیت بدنی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

4 استاد تمام مرکز تحقیقات اختلالات متابولیک، دانشگاه علوم پزشکی گلستان، گرگان، ایران

چکیده

مقدمه
شبکه اندوپلاسمی‎ در بسیاری از مسیرهای سلولی نقش اساسی دارد. تمرین منظم ورزشی و گرسنگی می‌توانند با فعال کردن مکانیسم‌هایی مانند ریتکولو‌فاژی منجر به سلامت کبد شوند. بنابراین هدف از تحقیق حاضر، بررسی تاثیر توام گرسنگی و تمرین هوازی بر شاخص‌های خطر زای، توده بدن و بیان ژن ATF6 شبکه اندوپلاسمی‎ کبد سالم رت‌های نر نژاد ویستار می‌باشد.
روش کار
در تحقیق تجربی حاضر تعداد 30 سر رت سالم نر نژاد ویستار18هفته‎ای ‎با دامنه وزنی 34/25 ±40/330 گرم انتخاب و به شش گروه 1)کنترل 2)گرسنگی3)3 روزتمرین 4)5 روزتمرین  5)گرسنگی+ 3 روزتمرین 6)گرسنگی+5 روزتمرین تقسیم شدند. رت‌های گروه‌ تمرینی، تمرین ورزشی را طی یک ماه،به تعداد 3 و 5 جلسه در هفته، به مدت یک ساعت روی تردمیل انجام دادند. گرسنگی به مدت 14ساعت در زمان بیداری رت‌ها و طی یک ماه به طور پیوسته انجام گردید. تجزیه و تحلیل داده‌ها با استفاده از آزمون‌های آماری آنوای‌ یک‌طرفه و تعقیبی LSD در نرم افزار SPSS نسخه 23 انجام گردید. سطح معنی داری 05/0≥P در نظر گرفته شد.
نتایج
تجزیه و تحلیل داده‌ها کاهش معنی‌داری در میزان وزن، BMI، LDL/HDL، CHOL/HDL، TAG/HDL و افزایش معنی‌داری در بیان ژن ATF6 نشان داد 05/0≥P .
نتیجه گیری
تمرین‌هوازی همراه با گرسنگی با کاهش شاخص‌های اتروژنیک منجر به سلامت کبد شد. همچنین افزایش بیان ژن ATF6 در کبد از طریق تمرین ورزشی دویدن و گرسنگی می‌تواند منجر به تنظیم ریتکولوم‌فاژی (خودخواری شبکه اندوپلاسمی) و سلامت کبد گردد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Combined Effect of Fasting and Aerobic Exercise on Atherogenic Indices, Body Mass And ATF6 Gene Expression in Healthy liver Endoplasmic Reticulum of Wistar Rats

نویسندگان [English]

  • Monireh Khalili 1
  • Habib Asgharpour 2
  • Asra Askari 3
  • Jahanbakhsh Asadi 4
1 Phd student of sports physiology, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 Assistant Professor of Physical Education and Sports Sciences, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
3 Assistant Professor of Sports Physiology, Department of Physical Education, Gorgan Branch, Islamic Azad University, Gorgan, Iran
4 Full Professor of Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
چکیده [English]

Introduction
Endoplasmic reticulum plays an essential role in many cellular pathways. Exercise and fasting can lead to liver health by activating mechanisms such as reticulophagy. Therefore, the aim of the current research is to  investigate the effect of fasting and aerobic exercise on atherogenic indicators, body mass and ATF6 gene expression in the endoplasmic reticulum of healthy liver of Wistar rats.
Material and Method
In this experimental research, 30 Wistar rats (18 weeks old) with a weight range of 330.40 ± 25.34 grams were selected and divided into six groups: 1) control 2) starvation 3) 3 days of training 4) 5 days of training 5) 3 Day of training + starvation  6) 5 days of training + starvation were divided. The rats in the training group trained on the treadmill for one month, 3 and 5 sessions a week for one hour. Starvation was for 14 hours when the rats were awake, which was done continuously for one month. Data analysis was performed using one-way ANOVA and post hoc LSD statistical tests in SPSS version 23 software. A significance level of P≥0.05 was considered.
Results
Data analysis showed a significant decrease in BMI, LDL/HDL, CHOL/HDL, TAG/HDL and a significant increase in ATF6 gene expression (P≥0.05).
Conclusion
Aerobic training with starvation led to liver health by reducing atherogenic indicators. Also, increasing the expression of ATF6 gene in the liver through exercise and starvation can lead to the regulation of reticulophagia and liver health.

کلیدواژه‌ها [English]

  • starvation
  • aerobic exercise
  • atherogenic
  • activator of transcription factor 6
  • endoplasmic reticulum

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 66، شماره 4
مهر-آبان1402
مهر و آبان 1402

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