بررسی تنوع ژنتیکی جدایه های مایکوباکتریوم توبرکلوزیس بیماران مبتلا به سل در کرج، ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه میکروبیولوژی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

2 گروه میکروب شناسی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد

3 گروه میکروبیولوژی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران. بخش تولید توبرکولین و مالئین، موسسه تحقیقات و سرم سازی کرج، کرج، ایران.

4 بخش تولید توبرکولین و مالئین، موسسه تحقیقات و سرم سازی کرج، کرج، ایران

5 گروه بهداشت حرفه‌ای، دانشکده بهداشت، مرکز تحقیقات بهداشت، ایمنی و محیط، دانشگاه علوم پزشکی البرز، کرج، ایران

چکیده

سابقه و هدف: سل معضلی قدیمی است که هم اکنون به عنوان چالشی جدید مطرح است. مطالعه حاضر با هدف بررسی تنوع ژنتیکی سویه های مایکوباکتریوم توبرکلوزیس جدا شده از بیماران مبتلا به سل در کرج، ایران انجام شد.
مواد و روش ها: هفده جدایه مایکوباکتریوم توبرکلوزیس از سال 2012 تا 2013 جمع‌آوری شد و تحت آنالیز پلی‌مورفیسم طول قطعه محدود IS6110 (IS6110-RFLP) قرار گرفتند. از نظر جمعیت شناختی، 6 زن و 14 مرد دارای تابعیت ایرانی در این مطالعه وارد شدند.
نتایج: 16 نوع ژنتیکی مختلف پس از هضم آنزیمی و تجزیه و تحلیل RFLP به دست آمد. تعداد کپی IS6110 در هر ایزوله از 0 تا 12 متغیر بود. اکثر ایزوله ها (66%) دارای اعداد کپی بین 6 تا 12 بودند. هر ایزوله دارای 6.9 کپی از نشانگر IS6110 بود. 9 جدایه دارای 10 تا 12 نسخه از نشانگر IS6110، 5 ایزوله دارای 6 تا 10 نسخه، و 2 ایزوله دیگر دارای نسخه های کمتر از 6 بودند. هیچ نسخه ای از IS6110 در بین 4 ایزوله یافت نشد. هیچ رابطه ای بین جنسیت و تعداد کپی یافت نشد.
نتیجه گیری: تنوع ژنتیکی بالای موجود در جدایه های مایکوباکتریوم توبرکلوزیس ممکن است منابع مختلف عفونت و اهمیت ظهور مجدد سل را نشان دهد.با این حال، بررسی های بیشتر باید برای ارزیابی سایر جنبه های اپیدمیولوژیک مولکولی مایکوباکتریوم توبرکلوزیس در ایران انجام شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Genetic diversity assessment of the Mycobacterium tuberculosis isolates of patients with tuberculosis in Karaj, Iran

نویسندگان [English]

  • Morassa Sadat Farnad 1
  • Hassan Momtaz 2
  • Keyvan Tadayon 3
  • Nader Mosavari 4
  • Yahya Khosravi 5
1 Department of Microbiology, Shahrekord Branch, Islamic Azad University, Sharekord, Iran
2 Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 Department of Microbiology, Shahrekord Branch, Islamic Azad University, Sharekord, Iran. Department of Tuberculin & Mallein, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
4 Department of Tuberculin & Mallein, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
5 Department of Occupational Health and Safety Engineering, Non-communicable Diseases, Research Center, Alborz University of Medical Sciences, Karaj, Iran.
چکیده [English]

Background and aim: Tuberculosis (TB) is an old issue that is presently measured as a significant challenge. The present survey was aimed to assess the genetic diversity of M. tuberculosis strains isolated from patients with TB in Karaj, Iran.
Methods: Seventeen M. tuberculosis isolates from 2012 to 2013 were collected and subjected to an IS6110 restriction fragment length polymorphism (IS6110-RFLP) analysis. Demographically, 6 females and 14 males who had Iranian citizenship were included in this study.
Results: Sixteen different genetic types were obtained after enzymatic digestion and RFL analysis. Copy numbers of IS6110 in each isolate ranged from 0 to 12. The majority of isolates (66%) harbored copy numbers between 6 and 12. Each isolates harbored 6.9 copies of the IS6110 marker. Nine isolates harbored 10 to 12 copies of the IS6110 marker, 5 isolates harbored 6 to 10 copies, and 2 others harbored copies less than 6. No copy of IS6110 was found among the 4 isolates. No relationship was found between gender and copy numbers.
Conclusion: The high genetic diversity found amongst the M. tuberculosis isolates maybe show different sources of infection and the importance of reemerging of the TB. However, further surveys should perform to assess other molecular epidemiologic aspects of M. tuberculosis in Iran.

کلیدواژه‌ها [English]

  • Mycobacterium tuberculosis
  • Genetic diversity
  • IS6110-RFLP
  • Iran

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 67، شماره 2
خرداد-تیر1403
خرداد و تیر 1403
صفحه 399-408

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