ارزیابی اثرات ضد دردی عصاره Eremostachys laevigata

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گیاهان داروئی؛ ادویه ایی و نوشابه ایی، گروه علوم باغبانی، واحد میانه، دانشگاه آزاد اسلامی، میانه، ایران

2 استادیار گروه علوم باغبانی، مرکز تحقیقات گیاهان دارویی و محصولات ارگانیک، واحد میانه، دانشگاه آزاد اسلامی، میانه، ایران(نویسنده مسول)

3 استادیار فارماکولوژی، تخصصی دامپزشکی، واحد میانه، دانشگاه آزاد اسلامی میانه

4 استادیار اکولوژی کشاورزی، زراعت، واحد میانه، دانشگاه آزاد اسلامی میانه، ایران.

چکیده

Eremostachys spp. به عنوان یک داروی گیاهی رایج در طب سنتی ایران با کاربردهای پزشکی ضد درد در نظر گرفته شده است. برای بررسی اثرات ضد دردی گیاه سنبل بیابانی رفیع ، عصاره‌های متانولی، آب مقطری، کلروفرمی، عصاره ان- هگزانی و استونی ریشه استخراج شده و 30 دقیقه قبل از درد ناشی از فرمالین، تزریق اسید استیک و روش صفحه داغ، در غلظت 100، 200، 300 و 400 میلی گرم بر کیلوگرم به موش تزریق شد. کنترل‌های مثبت و منفی به ترتیب مورفین و نرمال سالین بودند. غلظت ‌400 میلی گرم بر کیلوگرم عصاره ریشه گیاه می‌تواند به اندازه مورفین در تسکین درد ناشی از فرمالین مؤثر باشد. در آزمایش صفحه داغ و فرمالین، عصاره گیاهی 400 میلی گرم بر کیلوگرم می‌تواند درد را به اندازه مورفین تسکین دهد، اما در آزمایش اسید استیک تاثیر چندانی ندارد و این نشان می‌دهد که ممکن است از طریق سیستم عصبی مرکزی نسبت به سیستم محیطی عمل کند. میزان فنولیک با استفاده از روش فولین سیوکالتیو تعیین شد. کل فلاونوئیدها با روش رنگ سنجی کلرید آلومینیوم به روش اسپکتروفتومتری اندازه گیری شدند. مقدار کل فنولیک 87/6 میلی گرم بر گرم وزن خشک بود که براساس استاندارد اسید گالیک (GAE) و فلاونوئید 19/0 میلی گرم بر گرم وزن خشک براساس استاندارد روتین (RE) بیان شده بود. دو گلیکوزید ایریدوئیدی ضد درد، فلوئوزید I و سزاموزید با کمک HPLC مقدماتی فاز معکوس از ریشه این گیاه جدا گردیدند. بنابراین، عصاره متانولی ریشه E. laevigata می‌تواند کاندید مناسبی برای در نظر گرفتن بعنوان یک مسکن باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation Antinociceptive Effects of Eremostachys laevigata Extracts

نویسندگان [English]

  • parisa ahmadnia 1
  • Hassan Nourafcan 2
  • Assadi Assadi 3
  • Ali Faramarzi 4
1 PhD studentDepartment of Horticulture, College of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh,
2 Assistant Professor Department of Horticulture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
3 Assistant ProfessorDepartment of Veterinary medicine, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
4 Assistant Professor Department of Agronomy, College of Agriculture, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran
چکیده [English]

Eremostachys spp. have been considered as common herbal medicine in Iranian folk medicine, with pain relief medical applications. To investigate antinociceptive effects of Eremostachys laevigata, methanol, distilled water, chloroform, N-hexan and acetone extracts of root were extracted and injected to mice 30 minutes before pain induced by formalin, acid acetic injection and hot plate methods, in the concentrations of 100, 200, 300 and 400 mg/kg. The positive and negative controls were morphine and normal saline, respectively. Remarkable antinociceptive effect was observed in plant root methanol extract on the pain caused by formalin injection in both acute and chronic phases, visceral pain induced by acetic acid test, and increasing of pain tolerance duration in hot plate test. In hot plate and formalin test, the plant extract of 400 mg/kg could relief the pain as much as morphine, but not in acetic acid test, indicating that it may act through the central nervous than peripheral system. The phenolic content was determined using Folin-Ciocalteu assay. The total flavonoids were measured spectrophotometrically by the aluminium chloride colorimetric assay. The amount of total phenolic was 6.87 mg/g dry weight, expressed as gallic acid equivalents (GAE) and flavonoid was 0.19 mg/g dry weight expressed as rutin equivalents (RE). Two antipain iridoid glucosides, phloyoside I and Sesamoside have separated reversed-phase preparative HPLC from the root of this plant. Therefore, E. laevigata root methanol extract could be a suitable candidate for being considered as pain reliever.

کلیدواژه‌ها [English]

  • antipain
  • Chelle-Daghi
  • desert rod
  • pain reliever

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 67، شماره 1
شماره فروردین-اردیبهشت1403
فروردین و اردیبهشت 1403
صفحه 26-39

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