اثر هشت هفته تمرین هوازی همراه با مکمل ویتامین E بر برخی نروتروفین ها در بافت هیپوکامپ موش های صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، مرکز تحقیقات طب ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران

2 استادیار، مرکز تحقیقات طب ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران (نویسنده مسئول)

3 استادیار، مرکز تحقیقات طب ورزشی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران

چکیده

مقدمه: بیماری پارکینسون (PD) با اختلال در نروتروفین ها همراه است. مطالعات قبل تاثیر مطلوب تمرین و برخی آنتی اکسیدان ها را گزارش نموده اند. اما اثر همزمان آنها هنوز به خوبی شناخته نشده است. لذا هدف مطالعه حاضر بررسی اثر هشت هفته تمرین هوازی همراه با مکمل ویتامین E بر برخی نروتروفین ها در بافت هیپوکامپ موش های صحرایی مبتلا به پارکینسون بود.
روش کار:  در این مطالعه تجربی 40 سر موش صحرایی نر نژاد اسپراگو-داولی (سن 14-16 ماه و وزن 250 تا 270 گرم) پارکینسونی شده mg/kg 2 رزرپین (Rse) به گروه­های (1) pd، (2) AT، (3) VE و (4) AT+VE تقسیم شدند. جهت بررسی اثرات Res بر متغیر­ها تعداد 8 سر موش صحرایی در گروه کنترل سالم (HC) قرار گرفتند. تمرین هوازی به مدت هشت هفته، پنج جلسه در هفته و هر جلسه مدت 15-48 دقیقه با سرعت 10-24 متر بر دقیقه انجام شد. VE نیز روزانه mg/kg 30 به صورت خوراکی مصرف شد. برای تجزیه و تحلیل داده­ها از آزمون آنالیز واریانس یک­راهه و آزمون تعقیبی توکی استفاده شد (05/0≥P).
نتایج: مقادیر بیان ژنی BDNF و NGF در گروه های VE، AT و VE+AT به طور معنی داری بیشتر از گروه PD بود (001/0=P). همچنین افزایش این دو نورتروفین در گروه AT+VE به طور معنی داری بیشتر از گروه های VE و AT بود (001/0=P).
نتیجه گیری: با توجه به نتایج این مطالعه به نظر می رسد اگرچه تمرین هوازی و ویتامین E هر کدام به تنهایی موجب بهبود بیان نروتروفین ها می گردد. با این حال این دو مداخله می توانند از مسیر های مشترک اثر یکدیگر را در بیان نروتروفین ها در شرایط مدل سازی بیماری پارکینسون تقویت کنند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of eight weeks of aerobic exercise with vitamin E supplementation on some neurotrophins in the hippocampal tissue of rats with Parkinson's disease

نویسندگان [English]

  • Akram Rashidi 1
  • Jamshid Banaei Borojeni 2
  • Saeed Keshavarz 3
  • Elham Eftekhari Ghaeinani 3
1 PhD candidate, Sport Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran
2 Assistant professor, Sport Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran (Corresponding Author)
3 Assistant professor, Sport Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran
چکیده [English]

Introduction: Parkinson's disease (PD) is associated with disruption of neurotrophins. Previous studies have reported the favorable effect of exercise and some antioxidants. But their simultaneous effect is still not well known. Therefore, the aim of the present study was to investigate the effect of eight weeks of aerobic exercise with vitamin E supplementation on some neurotrophins in the hippocampal tissue of rats with Parkinson's disease.
Methods: In this experimental study, 40 Sprague-Dawley male rats (age 14-16 months and weight 250-270 grams) were given parkinsonism with 2 mg/kg reserpine (Rse) into groups (1) pd, ( 2) AT, (3) VE and (4) AT+VE were divided. In order to investigate the effects of Res on the variables, 8 rats were included in the healthy control group (HC). Aerobic training was done for eight weeks, five sessions a week and each session lasted 15-48 minutes at a speed of 10-24 meters per minute. VE was also taken orally at 30 mg/kg daily. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used (P≥0.05).
Findings: BDNF and NGF gene expression values in VE, AT and VE+AT groups were significantly higher than PD group (P=0.001). Also, the increase of these two nortrophins in the AT+VE group was significantly higher than the VE and AT groups (P=0.001).
Conclusion: According to the results of this study, it seems that although aerobic exercise and vitamin E alone improve the expression of neurotrophins. However, these two interventions can strengthen each other's effects in the expression of neurotrophins in the conditions of Parkinson's disease modeling.

کلیدواژه‌ها [English]

  • Exercise
  • Vitamin E
  • Neurotrophins
  • Hippocampus
  • Parkinson's Disease

مراجع

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مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
دوره 67، شماره 1
شماره فروردین-اردیبهشت1403
فروردین و اردیبهشت 1403
صفحه 375-385

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